The aim of this project is to introduce a fundamentally new concept for remote control of cellular functions by means of magnetic manipulation. The technology is based on magnetic nanoparticles functionalized with proteins involved in cellular signalling cascades. These bio-functionalized MNPs (bMNPs) will be delivered into target cells, where they act as intracellular signalling platforms activatable in a spatially and temporally controlled manner by external magnetic fields.
The key objectives of our proposal are:
- the development of the chemical, biochemical and physical tools for the magnetic control of signalling in stem cells and neuronal cells,
- the demonstration of magnetic actuation of differentiation and oriented growth of DA precursor neurons in single-cell in vitro assays
- the application of the technology with cells transplanted into organotypic brain slices and rodents.
By the end of the project, we will have reached a conceptual and practical expertise for the magnetic control of signalling activity in cells, with a demonstrated proof-of-concept for the differentiation and growth of stem cell derived neuronal cells in cultures, tissues and animals. This unique expertise will serve as foundation for subsequent applications of our technology in animal models and patients.
This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 686841.